TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description or genotype | Reference or source |
|---|---|---|
| Strains | ||
| E2348/69 | Wild-type EPEC strain, serotype O127:H6, produces α1 bundlin | 7 |
| UMD901 | E2348/69 bfpA C129S | 44 |
| RN587/1 | Wild-type EPEC strain, serotype O157:H+, produces β6 bundlin | 6 |
| UMD949 | RN587/1 bfpA C129S | This study |
| RN410/1 | Wild-type EPEC strain, serotype O119:H+, produces α3 bundlin | 7 |
| RN191/1 | Wild-type EPEC strain, serotype O111:H+, produces α2 bundlin | 7 |
| B171 | Wild-type EPEC strain, serotype O111:H+, produces α2 bundlin | 18 |
| E56/54 | Wild-type EPEC strain, serotype O128ab:H2, produces β4 bundlin | 7 |
| 012-050982 | Wild-type EPEC strain, serotype O142:H+, produces β1 bundlin | 7 |
| E851/71 | Wild-type EPEC strain, serotype O142:H6, produces β5 bundlin | 7 |
| Z188-93 | Wild-type avian EPEC strain, serotype O110:H6, produces β2 bundlin | 7 |
| CA89-4221 | Wild-type canine EPEC strain, serotype NT:H+, produces β3 bundlin | 7 |
| BL21(AI) slyD | F−ompT hsdSB (rB− mB−) gal dcm araB::T7RNAP-tetA ΔslyD::cat | 13 |
| Plasmids | ||
| pRPA100 | bfpA gene with native promoter cloned into low-copy-number vector pWKS30 | 3 |
| pXLW10 | pRPA100 with bfpA alpha-3 allele from strain RN410/1 | This study |
| pXLW11 | pRPA100 with bfpA alpha-2 allele from strain RN191/1 | This study |
| pXLW12 | pRPA100 with bfpA beta-4 allele from strain E56/54 | This study |
| pXLW13 | pRPA100 with bfpA beta-1 allele from strain 012-050982 | This study |
| pXLW14 | pRPA100 with bfpA beta-5 allele from strain E851/71 | This study |
| pXLW15 | pRPA100 with bfpA beta-6 allele from strain RN587/1 | This study |
| pXLW16 | pRPA100 with bfpA beta-2 allele from strain Z188-93 | This study |
| pXLW17 | pRPA100 with bfpA beta-3 allele from strain CA89-4221 | This study |
| pIL14 | AFA-1 afimbrial adhesin genes cloned in a pBR322 vector | 23 |
| pPF301 | Expression vector for soluble α1 bundlin under control of T7 promoter | 34 |
| pPF302 | Expression vector for soluble β6 bundlin under control of T7 promoter | This study |
| pKEN | pUC derivative encoding eGFP | 11 |
| pTR103 | pBluescript derivative with eGFP under control of consensus sigma-70 promoter | This study |
| pDsRed | Red fluorescent protein under control of lac promoter in pUC19 backbone | Clonetech |