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Immunodetection of PAR in infected A431 cells. A431 cells were mock infected with medium (A), infected with wt MS11 (B) or MS11pilT (C) for 5.5 h, or treated with STS for 4.5 h (D). Samples were fixed and stained with antibodies to PAR (green) and DAPI (blue) to visualize nuclei. All images were acquired on a Nikon Microphot-FX at the same magnification. PAR and DAPI signals are superimposed in all images.
