FIG. 4.
Multimeric LFN protects against LFN-DTA effects on zebrafish vasculature. Zebrafish embryos expressing EGFP under the control of a fli-1 promoter [Tg(fli1:EGFP)y1] (11) were injected with PA, LFN-DTA, and/or 68.5 ng of inhibitor as indicated (representative embryos are depicted; n = 20). Photographs of the same embryos were taken for each pair of phase-contrast (left panels) and fluorescence (right panels) micrographs. Loss of fluorescence in LFN-DTA- and PA-injected panels indicates endothelial cell death.