FIG. 4.

Immunoreactivities of recombinant and synthetic overlapping 14- and 15-mer peptides (Table 3) spanning the epitope-containing regions of E. canis gp200. Positive control (Ctrl or +), E. chaffeensis p28-19 peptide epitope; lane and x-axis numbers correspond to the peptide numbers in Table 3. (A) Membrane total protein staining. (B) Western immunoblot probed with polyclonal dog anti-E. canis serum. (C) Immunoreactivities of the recombinant gp200 peptides and the corresponding synthetic gp200 peptides expressed as the average optical densities at 650 nm of quadruplet samples as determined by ELISA. The recombinant E. canis gp200 regions corresponding to the overlapping peptides are labeled under the x axis. Molecular masses (kilodaltons) are shown on the left.