TABLE 1.

Primers and optimal annealing temperature used for the real-time PCR analysis of sagA, siaA, and gyrA

Gene	Optimal annealing temp (°C)	Forward primer	Reverse primer
sagA	50	5′-AGGAGGTAAACCTTATGTTA-3′	5′-TACCACCTTGAGAATTACCA-3′
siaA	65	5′-CAGCAGAGAATTGTAGCCACTTCG-3′	5′-CCCACACGCTTAACAGCATCATAG-3′
gyrA	65	5′-AGCGAGACAGATGTCATTGCTCAG-3′	5′-CCAGTCAAACGACGCAAACG-3′