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Inability to detect PNAG in S. lugdunensis extracts by immunoblotting. Extracts from 24-h biofilm cells grown in TSBgluc1% were immunoblotted with an antibody raised against deacetylated PNAG. Cells were boiled in 0.5 M EDTA, and the supernatants were treated with proteinase K prior to blotting on nitrocellulose.
