Table 1.
Time (min) | Flow (ml/min) | %Buffer Aa | %Buffer Bb | Curve |
---|---|---|---|---|
Initial | 0.80 | 100 | 0 | - |
3.00 | 0.80 | 100 | 0 | 5 |
43.00 | 0.80 | 80 | 20 | 5 |
53.00 | 0.80 | 30 | 70 | 5 |
55.00 | 0.80 | 30 | 70 | 5 |
60.00 | 0.80 | 100 | 0 | 5 |
90.00 | 0.80 | 100 | 0 | 9 |
Buffer A: 0.1% trifluoroacetic acid (TFA) in deionized water.
Buffer B: 90% acetonitrile and 0.098% TFA in deionized water.