Table 1.
Conditions for separation of adenosine- and diadenosine phosphates by reverse phase high performance liquid chromatography (RP-HPLC) at low pH using volatile organic buffers
| Time (min) | Flow (ml/min) | %Buffer Aa | %Buffer Bb | Curve |
|---|---|---|---|---|
| Initial | 0.80 | 100 | 0 | - |
| 3.00 | 0.80 | 100 | 0 | 5 |
| 43.00 | 0.80 | 80 | 20 | 5 |
| 53.00 | 0.80 | 30 | 70 | 5 |
| 55.00 | 0.80 | 30 | 70 | 5 |
| 60.00 | 0.80 | 100 | 0 | 5 |
| 90.00 | 0.80 | 100 | 0 | 9 |
a
Buffer A: 0.1% trifluoroacetic acid (TFA) in deionized water.
b
Buffer B: 90% acetonitrile and 0.098% TFA in deionized water.