Fig. 2. TAC-induced cardiac JNK activation is reduced in dominant negative mice and gene-targeted mice. (A) Autoradiograph of ³²P-labeled GST-cJun (1–79) phosphorylation from heart protein extracts of the indicated mice at baseline or 7 days after TAC stimulation (some lanes were spliced together for simplicity). (B) Quantitative analysis of JNK activity in each of the indicated groups (N = 6 each) (*P < 0.05 versus sham; †P < 0.05 versus C57BL/6 or FVB WT TAC). (C) Time course of cJun kinase activity in wild-type (WT) or JNK1/2 dn mice following TAC stimulation.