Fig. 6. Depletion of clathrin heavy chain in procyclic culture form (PCF) cells leads to a rounded morphology. (A) Gallery of phase images from PCF cultures expressing clathrin RNAi. The leftmost image shows the ‘normal’ PCF phenotype, the next two images show the ‘fat’ phenotype, and the three images on the right show the ‘round’ phenotype. (B) Incidence of aberrant cell morphologies in induced PCF clathrin RNAi cell cultures. Blue bars, uninduced PCFp2T7^TiCLH cells cultured for 48 h (dark blue) or 72 h (pale blue); red bars, induced PCFp2T7^TiCLH cultures at 48 h (dark red) or 72 h (pale red). Morphologies were defined as follows: normal, average cell body length 15 µm, average width 1.54 µm; long, cell body length >20 µm, average cell body length 23.3 µm, average width 1.54 µm; fat, enlarged posterior of the cell, average cell body length 8.55 µm, average width 3.07 µm; round, cell body completely rounded up, with detached or no visible flagellum, average cell body length 4.66 µm, average width 4.26 µm; other, all other abnormal forms.