Fig. 2. Mapping AD2 within the 552–705 region. The indicated regions of Zap1 were fused to the GBD and expressed in gal4Δ cells (ABY29) co-transformed with the GAL1-lacZ reporter. Cells were grown to exponential phase in low zinc conditions (LZM + 3 µM ZnCl₂) prior to β-galactosidase activity assays. A representative experiment is shown and each value is the mean of three replicates. Error bars represent 1 SD.