Fig. 6. Strain ABY29 (gal4Δ) was transformed with the GAL1-lacZ reporter and plasmids expressing the indicated ZAP1 mutations in the GBD–Zap1_552–705 fusion protein or the vector-only control. These cells were grown to exponential phase in LZM medium plus the indicated concentration of ZnCl₂. Representative experiments are shown and each value is the mean of three replicates. Error bars represent ±1 SD.