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| His-PAP (ng)a | GST–14-3-3ε (ng)a | Relative PAP activity |
|---|---|---|
| 50 | 0 | 1.00 |
| 50 | 10 | 0.89 ± 0.08 |
| 50 | 30 | 0.75 ± 0.04 |
| 50 | 100 | 0.70 ± 0.08 |
| 50 | 300 | 0.62 ± 0.02 |
aHis-PAP and GST–14-3-3ε were purified as recombinant proteins from Sf9 cells and E.coli cells, respectively. The polyadenylation reaction was carried out as in Figure 6A, and the reaction products were analyzed by the trichloroacetic acid precipitation assay.
