Fig. 5. MO25α and STRADα anchor LKB1 in the cytoplasm. HeLa cells were transfected with the indicated constructs encoding for the expression of Myc-MO25α, Flag-STRADα and GFP–LKB1. Twenty-four hours post-transfection the cells were fixed in 4% (by vol) paraformaldehyde and immunostained with anti-MO25α antibody to detect MO25α (TR anti-sheep secondary antibody, red channel) and anti-Flag to detect STRADα (Cy5 anti-mouse secondary antibody, blue channel). GFP–LKB1 localization was visualized directly through the GFP fluorescence (green channel). The cells were imaged using a Zeiss LSM 510 META confocal microscope. The cells shown are representative images obtained in three separate experiments. The scale bars correspond to 10 µm.