Fig. 2. Lsh does not regulate global histone methylation levels. (A) Loss of Lsh does not alter H3-K9me, H3-K4me or Hp1 protein levels. Western analysis of nuclear extracts derived from four Lsh-deficient embryos (gestation day 14) and their wild-type littermates. (B) Titration of nuclear extracts of one wild-type and one Lsh-deficient embryo. (C) Loss of Lsh does not alter Hp1 binding to chromatin. Lsh-deficient and Lsh wild-type MEFs are compared for resistance to Triton X-100 washes followed by western analysis to detect HP1 or PCNA and Vimentin as control.