Skip to main content
. 2003 Oct 1;22(19):4922–4932. doi: 10.1093/emboj/cdg494

Table II. AbfA kinetic parameters for the hydrolysis of aryl-α-l-arabinofuranosides and aryl-β-d-xylopyranosidesa.

Phenol substituent (pKa) Glyconb kcat (s–1) Ratio kcat [AF/Xyl] Km (mM) Ratio Km [AF/Xyl] kcat/Km (s–1 mM–1) Ratio kcat/Km [AF/Xyl]
2,5-Dinitro (5.15) AF 190 2.6 0.35 1/23 540 60
  Xyl 74   8.0   9.3  
3,4-Dinitro (5.36) AF 340 34 0.53 1/8 640 280
  Xyl 10   4.4   2.3  
4-Nitro (7.18) AF 87 4.3 0.65 1/23 130 100
2-Nitro (7.22) Xyl 20   15.3   1.3  

aValues taken from Shallom et al. (2002a,b).

bAF, arabinofuranosyl; Xyl, xylopyranosyl.