Table II. AbfA kinetic parameters for the hydrolysis of aryl-α-l-arabinofuranosides and aryl-β-d-xylopyranosidesa.
Phenol substituent (pKa) | Glyconb | kcat (s–1) | Ratio kcat [AF/Xyl] | Km (mM) | Ratio Km [AF/Xyl] | kcat/Km (s–1 mM–1) | Ratio kcat/Km [AF/Xyl] |
---|---|---|---|---|---|---|---|
2,5-Dinitro (5.15) | AF | 190 | 2.6 | 0.35 | 1/23 | 540 | 60 |
Xyl | 74 | 8.0 | 9.3 | ||||
3,4-Dinitro (5.36) | AF | 340 | 34 | 0.53 | 1/8 | 640 | 280 |
Xyl | 10 | 4.4 | 2.3 | ||||
4-Nitro (7.18) | AF | 87 | 4.3 | 0.65 | 1/23 | 130 | 100 |
2-Nitro (7.22) | Xyl | 20 | 15.3 | 1.3 |
aValues taken from Shallom et al. (2002a,b).
bAF, arabinofuranosyl; Xyl, xylopyranosyl.