Figure 3.
hFVIII-dependent proliferation of mouse CD4+ splenocytes derived from the two vector groups 35 wk posttransplant. Incorporation of [3H]thymidine into CD4+ splenocyte DNA was measured for the LNL6 group (open symbols) and for the GCsamF8EN group, low titer anti-FVIII antibody responders (filled symbols), after culture for 4½ days in the presence of purified B domain-deleted hFVIII. Animals were from two independent BM transplant experiments. The stimulation index represents the incorporation of 3H with the indicated concentration of FVIII divided by control incorporation with no FVIII for the same T cell source. The control values (average ± SD) were: LNL6, 14,232 ± 7,115 cpm and GCsamF8EN, 26,699 ± 13,265 cpm. Accessory cells alone gave <100 cpm. Data are presented as average stimulation index ± SD for n = 6 (LNL6) and n = 4 (GCsamF8EN).