Table I. Data collection, phasing, and refinement.
| Data collection | RecQΔC | ATPγS-bound RecQΔC | Phasing statistics | Refinement | RecQΔC | ATPγS-bound RecQΔC | |
|---|---|---|---|---|---|---|---|
| Wavelength (Å) | 0.9791 | 0.9000 | Figure of merit | 0.293 | Resolution (Å) | 20.0–1.8 | 20.0–2.5 |
| Resolution (last shell) (Å) | 74–1.8 (1.89–1.8) | 21–2.5 (2.63–2.5) | (after density modification) | 0.809 | Rwork/Rfreeb | 19.3/24.4 | 21.0/29.8 |
| Measured reflections/unique | 353 669/51 478 | 47 206/17 529 | R.m.s.d. bond lengths (Å) | 0.006 | 0.012 | ||
| Rsyma (last shell) (%) | 8.1 (44.2) | 4.8 (13.3) | R.m.s.d. bond angles (°) | 1.7 | 3.2 | ||
| I/σ (last shell) | 16.3 (4.6) | 12.8 (7.1) | PDB code | 1OYW | 1OYY | ||
| Completeness (last shell) (%) | 99.7 (99.7) | 92.1 (92.1) |
aRsym = ΣΣj|Ij – <I>|/ΣIj, where Ij is the intensity measurement for reflection j and <I> is the mean intensity for multiply recorded reflections.
bRwork, free = Σ||Fobs| – |Fcalc||/|Fobs|, where the working and free R-factors are calculated using the working and free reflection sets, respectively. The free reflections (5% of the total) were held aside throughout refinement.