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. 1983 May;79(1):63–70. doi: 10.1111/j.1476-5381.1983.tb10496.x

The binding of [125I]-angiotensin to rat renal epithelial cell membranes.

H M Cox, K A Munday, J A Poat
PMCID: PMC2044835  PMID: 6307453

Abstract

1 Specific high affinity binding sites for [125I]-angiotensin II have been identified in crude basolateral and brush border membranes from rat cortex. 2 A central high affinity site, KD 0.62 nM; Bmax 299 fmol/mg was identified as part of a complex multicomponent binding system. 3 This high affinity site was saturable and exhibited specificity for angiotensin II analogues and closely related peptides but not for bradykinin, substance P or peptide fragments of angiotensin II. 4 Specific [125I]-angiotensin II binding was partially dependent on NaCl. Absence of NaCl resulted in a decrease in Bmax, had no effect on the rate of association but increased the rate of dissociation of [125I]-angiotensin from its binding site.

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Selected References

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