Table II. Kinetic parameters for clathrin interactions determined by SPR.

Immobilized protein/mobile protein	Concentration of immobilized species	Concentration threshold for binding	kdiss
Proximal leg/proximal leg	0.92 mM	1.0–2.5 µM	0.012
Hub/Hub	0.28 mM	100–500 nM	0.0026
Proximal-Ii/proximal-Ii	0.30 mM	250–500 nM	0.0051
Distal-Ii/distal-Ii	0.24 mM	100–250 nM	0.0048
Hub/distal-Ii	0.28 mM	100–250 nM	0.0018