Skip to main content
. Author manuscript; available in PMC: 2008 Oct 1.
Published in final edited form as: DNA Repair (Amst). 2007 Jun 4;6(10):1496–1506. doi: 10.1016/j.dnarep.2007.04.012

Fig. 1. Salt-sensitivity of Rad51-rad54 Δ129 in chromatin remodeling.

Fig. 1

(A) MNase (5 units/ml in lanes 2 and 5, 10 units/ml in lanes 3 and 6, and 15 units/ml in lane 7) analysis of free (lanes 1-3) and chromatinized (lanes 4-7) DNA.

(B) The ability of Rad54 (wt) or rad54 Δ129 (Δ129) to render the chromatinized DNA template sensitive to the restriction enzyme HaeIII was examined as a function of the KCl concentration. The analysis was conducted with or without Rad51, as indicated. Naked DNA was used in lanes 2 and 3 and chromatinized DNA was used in lanes 4 to 24. Size markers were run in lane 1. HaeIII was added to all the other reactions except those in lanes 2 and 4.

(C) Chromatin remodeling by Rad54 or rad54 Δ129 at various KCl concentrations (25, 50, and 80 mM).

(D) Chromatin remodeling by Rad54 with or without Rad51 or RecA, as indicated.