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. Author manuscript; available in PMC: 2008 Oct 1.
Published in final edited form as: DNA Repair (Amst). 2007 Jun 4;6(10):1496–1506. doi: 10.1016/j.dnarep.2007.04.012

Fig. 3. Enhancement of Rad54 ATPase activity by rad51 K191A.

Fig. 3

(A) Rad51, rad51 K191A (K191A), and rad51 K191R (K191R) were incubated with His-tagged Rad54. Protein complexes were captured on nickel NTA agarose beads and then eluted with SDS. The supernatant (S), wash (W), and SDS-eluate (E) were analyzed by SDS-PAGE.

(B) ATP hydrolysis by Rad54 was examined with or without Rad51, rad51 K191A, and rad51 K191R.