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. Author manuscript; available in PMC: 2008 Oct 1.
Published in final edited form as: Cancer Res. 2007 Oct 1;67(19):9463–9471. doi: 10.1158/0008-5472.CAN-07-2034

Figure 4.

Figure 4

Glutamate released through system xC caused glioma Ca2+ oscillations. A, increasing amounts of the cystine were added to three glioma cell lines, thus inducing the release of glutamate in a dose-dependent manner as determined by the highly sensitive enzyme-based bioluminescent assay. B, FURA2-AM–loaded D54-MG cells are shown in a representative field before measuring calcium oscillations at ×40 magnification. C, three representative cells from three coverslips were imaged every 15 s in the presence of cystine or with cystine but in the presence of one of the two system xC inhibitors, sulfasalazine (SAS) or S-(4)-CPG.

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