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. 1993 Apr;175(8):2229–2235. doi: 10.1128/jb.175.8.2229-2235.1993

Adverse conditions which cause lack of flagella in Escherichia coli.

C Li 1, C J Louise 1, W Shi 1, J Adler 1
PMCID: PMC204508  PMID: 8385664

Abstract

Wild-type Escherichia coli was not motile when grown in tryptone broth under the following adverse conditions: the presence of high temperature [J. Adler and B. Templeton, J. Gen. Microbiol. 46:175-184, 1967; R. B. Morrison and J. McCapra, Nature (London) 192:774-776, 1961; K. Ogiuti, Jpn. J. Exp. Med. 14:19-28, 1936], high concentrations of salts, high concentrations of carbohydrates, high concentrations of low-molecular-weight alcohols, or the pressure of gyrase inhibitors. Under all these conditions, growth was necessary for the loss of motility. This loss of motility was correlated with a reduction in the amount of cellular flagellin. We isolated and studied mutants that are resistant to suppression of motility by some of these conditions, because of the ability to synthesize flagella under these conditions. The mutations were mapped to 42 min, a region of the chromosome where many of the flagellar genes map. We also studied the effect of a preexisting gyrA mutation which allowed flagellar formation in the presence of nalidixate.

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Selected References

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  1. Adler J. Chemotaxis in bacteria. Science. 1966 Aug 12;153(3737):708–716. doi: 10.1126/science.153.3737.708. [DOI] [PubMed] [Google Scholar]
  2. Adler J., Hazelbauer G. L., Dahl M. M. Chemotaxis toward sugars in Escherichia coli. J Bacteriol. 1973 Sep;115(3):824–847. doi: 10.1128/jb.115.3.824-847.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Adler J., Li C., Boileau A. J., Qi Y., Kung C. Osmotaxis in Escherichia coli. Cold Spring Harb Symp Quant Biol. 1988;53(Pt 1):19–22. doi: 10.1101/sqb.1988.053.01.005. [DOI] [PubMed] [Google Scholar]
  4. Adler J., Templeton B. The effect of environmental conditions on the motility of Escherichia coli. J Gen Microbiol. 1967 Feb;46(2):175–184. doi: 10.1099/00221287-46-2-175. [DOI] [PubMed] [Google Scholar]
  5. Armstrong J. B., Adler J., Dahl M. M. Nonchemotactic mutants of Escherichia coli. J Bacteriol. 1967 Jan;93(1):390–398. doi: 10.1128/jb.93.1.390-398.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bachmann B. J. Linkage map of Escherichia coli K-12, edition 8. Microbiol Rev. 1990 Jun;54(2):130–197. doi: 10.1128/mr.54.2.130-197.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Bingham R. J., Hall K. S., Slonczewski J. L. Alkaline induction of a novel gene locus, alx, in Escherichia coli. J Bacteriol. 1990 Apr;172(4):2184–2186. doi: 10.1128/jb.172.4.2184-2186.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Drlica K., Franco R. J. Inhibitors of DNA topoisomerases. Biochemistry. 1988 Apr 5;27(7):2253–2259. doi: 10.1021/bi00407a001. [DOI] [PubMed] [Google Scholar]
  9. Edelmann P., Gallant J. Mistranslation in E. coli. Cell. 1977 Jan;10(1):131–137. doi: 10.1016/0092-8674(77)90147-7. [DOI] [PubMed] [Google Scholar]
  10. Farr S. B., Kogoma T. Oxidative stress responses in Escherichia coli and Salmonella typhimurium. Microbiol Rev. 1991 Dec;55(4):561–585. doi: 10.1128/mr.55.4.561-585.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hane M. W., Wood T. H. Escherichia coli K-12 mutants resistant to nalidixic acid: genetic mapping and dominance studies. J Bacteriol. 1969 Jul;99(1):238–241. doi: 10.1128/jb.99.1.238-241.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Komeda Y. Fusions of flagellar operons to lactose genes on a mu lac bacteriophage. J Bacteriol. 1982 Apr;150(1):16–26. doi: 10.1128/jb.150.1.16-26.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  14. Li C., Boileau A. J., Kung C., Adler J. Osmotaxis in Escherichia coli. Proc Natl Acad Sci U S A. 1988 Dec;85(24):9451–9455. doi: 10.1073/pnas.85.24.9451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Macnab R. M., Parkinson J. S. Genetic analysis of the bacterial flagellum. Trends Genet. 1991 Jun;7(6):196–200. doi: 10.1016/0168-9525(91)90436-t. [DOI] [PubMed] [Google Scholar]
  16. Sager B. M., Sekelsky J. J., Matsumura P., Adler J. Use of a computer to assay motility in bacteria. Anal Biochem. 1988 Sep;173(2):271–277. doi: 10.1016/0003-2697(88)90189-3. [DOI] [PubMed] [Google Scholar]
  17. Shen L. L., Pernet A. G. Mechanism of inhibition of DNA gyrase by analogues of nalidixic acid: the target of the drugs is DNA. Proc Natl Acad Sci U S A. 1985 Jan;82(2):307–311. doi: 10.1073/pnas.82.2.307. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Shi W., Li C., Louise C. J., Adler J. Mechanism of adverse conditions causing lack of flagella in Escherichia coli. J Bacteriol. 1993 Apr;175(8):2236–2240. doi: 10.1128/jb.175.8.2236-2240.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Silverman M., Simon M. I. Bacterial flagella. Annu Rev Microbiol. 1977;31:397–419. doi: 10.1146/annurev.mi.31.100177.002145. [DOI] [PubMed] [Google Scholar]
  20. Silverman M., Simon M. Characterization of Escherichia coli flagellar mutants that are insensitive to catabolite repression. J Bacteriol. 1974 Dec;120(3):1196–1203. doi: 10.1128/jb.120.3.1196-1203.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Singer M., Baker T. A., Schnitzler G., Deischel S. M., Goel M., Dove W., Jaacks K. J., Grossman A. D., Erickson J. W., Gross C. A. A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli. Microbiol Rev. 1989 Mar;53(1):1–24. doi: 10.1128/mr.53.1.1-24.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Slonczewski J. L., Gonzalez T. N., Bartholomew F. M., Holt N. J. Mu d-directed lacZ fusions regulated by low pH in Escherichia coli. J Bacteriol. 1987 Jul;169(7):3001–3006. doi: 10.1128/jb.169.7.3001-3006.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Smith P. K., Krohn R. I., Hermanson G. T., Mallia A. K., Gartner F. H., Provenzano M. D., Fujimoto E. K., Goeke N. M., Olson B. J., Klenk D. C. Measurement of protein using bicinchoninic acid. Anal Biochem. 1985 Oct;150(1):76–85. doi: 10.1016/0003-2697(85)90442-7. [DOI] [PubMed] [Google Scholar]
  24. Smith R. A., Parkinson J. S. Overlapping genes at the cheA locus of Escherichia coli. Proc Natl Acad Sci U S A. 1980 Sep;77(9):5370–5374. doi: 10.1073/pnas.77.9.5370. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Sugino A., Peebles C. L., Kreuzer K. N., Cozzarelli N. R. Mechanism of action of nalidixic acid: purification of Escherichia coli nalA gene product and its relationship to DNA gyrase and a novel nicking-closing enzyme. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4767–4771. doi: 10.1073/pnas.74.11.4767. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Tso W. W., Adler J. Negative chemotaxis in Escherichia coli. J Bacteriol. 1974 May;118(2):560–576. doi: 10.1128/jb.118.2.560-576.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

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