Figure 4.

HGF increases p21 in MDCK cells. a. MDCK cells were seeded and serum starved for 24 h. HGF (20 and 40 ng/ml) was added for 24 h in serum-free quiescence media and the cells were lysed and immunoblotted with anti-p21 and β-actin antibodies. Human p21 was used for p21 positive control and indicates that lower band of doublet is p21. QM: quiescence media; p21 pos: p21 positive control (HeLa cell lysate). Densitometry is shown; *p < 0.05 as compared to QM alone. Experiment shown is representative of two experiments. b. In order to determine which band of the doublet represents p21, MDCK and HeLa control cell lysate were immunoblotted with p21 antibody alone or with p21 antibody which had been pre-incubated with GST- tagged p21 antigen, demonstrating that the lower molecular weight band is p21.