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Schematic representation of plasmids. The open boxes represent the ORFs of β-lactamase (bla) and chloramphenicol acetyl transferase (cat) genes. The solid boxes correspond to the ORF of cyaA′, with codon numbers indicated below. The hatched boxes correspond to the multicloning site sequences (MCS) that are fused at the indicated position of the cya ORF. The origin of replication of the plasmids is indicated by shaded boxes.
