Table 1.
Analysis of complementation in DHP1 strain
| Plasmids | Phenotype on MacConkey/maltose | β-Galactosidase, units/mg dry weight bacteria | cAMP, pmol/mg dry weight bacteria | Adenylate cyclase activity*
|
|
|---|---|---|---|---|---|
| +CaM | −CaM | ||||
| None | White | 179 | <10 | <1 | <0.01 |
| pCm-AHL1 | Red/24 hr | 6,650 | 3,400 | 13,000 | 10 |
| pT25 + pT18 | White/72 hr | 130 | <10 | <1 | <0.01 |
| pT25 + pT18-zip | White/72 hr | 183 | <10 | <1 | <0.01 |
| pT25-zip + pT18 | White/72 hr | 178 | <10 | <1 | <0.01 |
| pT25-zip + pT18-zip | Red/30 hr | 4,750 | 1,100 | 10,000 | 4 |
Bacteria were grown in LB at 30°C in the presence of 0.5 mM isopropyl β-d-thiogalactoside plus appropriate antibiotics. The results represent the average values obtained for at least five independent cultures, which differed by less than 10%.
*
nmol cAMP/min per mg protein; when present in the assays, CaM was at a concentration of 1 μM.