Table 2.
Complementation between various chimeric proteins
| Plasmids | Phenotype on MacConkey/maltose | β-Galactosidase, units/mg dry weight bacteria | cAMP, pmol/mg dry weight bacteria |
|---|---|---|---|
| pT25-Tyr + pT18-Tyr | Red/40 hr | 2,800 | 580 |
| pT25-Tyr + pT18 | White/96 hr | 193 | <10 |
| pT25 + pT18-Tyr | White/96 hr | 183 | <10 |
| pT25-Tyr + pT18-zip | White/96 hr | 134 | <10 |
| pT25-zip + pT18-Tyr | White/96 hr | 126 | <10 |
| pT25-prp11 + pT18-prp21 | Red/40 hr | 850 | 65 |
Bacteria were grown in LB at 30°C in the presence of 0.5 mM isopropyl β-d-thiogalactoside plus appropriate antibiotics. The results represent the average values obtained for at least five independent cultures.