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. Author manuscript; available in PMC: 2007 Nov 28.
Published in final edited form as: J Biol Chem. 2005 Dec 27;281(8):4739–4745. doi: 10.1074/jbc.M509042200

Table 1.

Parameters of tocopherol absorption as a function of micellar tocopherol concentrations in differentiated Caco-2 TC-7 cell monolayers.

Vitamin E species Apparent Qmax (nmole tocopherol absorbed/mg of protein) Apparent K (μM)
RRR-α-T 7.5 ± 0.6 84.3 ± 9.9
RRR-γ-T 6.5 ± 0.5 83.9 ± 11.5
DL-α-T 5.6 ± 0.7 88.3 ± 15.2

Tocopherol uptake was measured at 37°C after apical micellar tocopherol delivery in increasing concentrations. Incubation time was 15 min. Best fitting curves were hyperbolic curves y = ax/(b+x). Qmax represents the maximal amount of tocopherol absorbed, and K represents the concentration of micellar tocopherol required to reach Qmax/2. Data are means ± SD of 3 assays.