Fig. 2.

Response of the yeast androgen biosensor to different substances. Exposure to 17β-testosterone, 5α-dihydrotestosterone, progesterone, dexamethasone, 17β-estradiol, and 17β-boldenone was started by adding to 200 μL of a yeast culture a 2-μL aliquot of a stock solution of the compound in dimethyl sulfoxide (DMSO). Fluorescence was determined after 24 h as described in “Streamlined yEGFP assay with the yeast androgen bioassay.” Fluorescence signals are the mean of a triplicate with the standard deviation (SD). 17β-T 17β-testosterone, DHT 5α-dihydrotestosterone, Prog progesterone, Dex dexamethasone, 17β-E2 17β-estradiol, Bold 17β-boldenone