Figure 3.

Increased Meis1 expression accelerates MLL-mediated leukemogenesis. (A) Meis1 transcript levels in myeloid progenitors (c-kit⁺) were determined by quantitative real-time PCR 24 h following transduction with retroviruses encoding the MLL oncogenes indicated above the panel, with or without cotransduced Meis1 (indicated below). Data are expressed relative to the level observed in cells transduced with MLL oncogene alone (bars indicate the average of triplicate analyses). (B) Survival curves are shown for cohorts of mice transplanted with cells cotransduced with the indicated MLL oncogene and Meis1 or empty vector. Acute leukemia was confirmed by peripheral blood leukocyte counts, FACS analyses, and/or necropsy.