Skip to main content
. Author manuscript; available in PMC: 2007 Oct 30.
Published in final edited form as: Pain. 2007 Apr 24;130(1-2):166–176. doi: 10.1016/j.pain.2007.03.012

Figure 6.

Figure 6

Depletion of dural mast cell granules blocks 48/80-induced fos expression in the TNC. (A and B) Samples of TB-stained dura mater taken from an animal undergoing a unilateral craniotomy 24 hours earlier. Note that on the craniotomized side (A) there is a complete loss of TB-staining of dural MC indicating granule depletion while the intact, non-craniotomized side (B) shows the typical level of MC degranulation following 48/80 treatment. (C) Cameral Lucida reconstruction of the anatomical location of fos-IR cells within the superficial dorsal horn (laminae I and II) at the level of the caudal TNC following MC degranulation in an animal that underwent a unilateral MC depletion using craniotomy. Each drawn section plots the location of fos-IR cells from 3 consecutive, alternate, 40 mm sections. Note that dural MC depletion blocked fos-IR only in the TNC ipsilateral to the depleted side. (D) Plot of the rostrocaudal distribution of the mean number of fos-IR cells in the dorsal horn ipsi- and contralateral to the MC depleted side in animals injected with 48/80 (n=4). Asterisks indicate a significant difference between the ipsi- and contralateral sides (p<0.05, Fisher PLSD test). Scale bar = 200 μm.