TABLE 2.
Specific activities of NADH-generating enzymes in cells harvested from menadione-containing and control media at the same growth phase (25 h for control cells and 30 h for menadione-stressed cells)a
NAD+-dependent enzyme | Sp act (nmol min−1 mg protein−1)a
|
|
---|---|---|
Control | Menadione stressed | |
ICDH-NAD+ | 55 ± 5 | 15 ± 3* |
a-KGDH | 40 ± 4 | 7 ± 1* |
GDH-NAD+ | 50 ± 7 | 12 ± 3* |
PDH | 6 ± 0.3 | 2 ± 0.2 |
MDH | 11 ± 1 | 13 ± 1* |
The level of NADH was monitored. The values are means ± standard deviations. An asterisk indicates that the P value is ≤0.05 (n = 3).