FIG. 3.

Insertion of a hygromycin resistance cassette into sirA and Rv2393 in M. smegmatis mc²155. (A) Primer sets used for the construction of Southern blot probes and AESs. (B) Southern blot of genomic DNAs from wild-type (wt) and ΔsirA strains. BanII-digested genomic DNA was probed using a PCR fragment generated using primers 1 and 1′. The blot revealed that insertion of the resistance cassette caused the expected shift from 1.1 to 2.5 kb. (C) Southern blot of genomic DNAs from the wild-type (wt) and ΔRv2393 strains. MscI-digested genomic DNA was probed using a PCR fragment generated using primers 4 and 4′. Insertion of the resistance cassette resulted in the expected shift from 6.3 to 5.0 kb.