TABLE 1.
Bacterial strains used in this study
| Strain | Description | Source or reference |
|---|---|---|
| E. coli strains | ||
| XL1-Blue | recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10 (Tetr)] | Stratagene |
| BL21(DE3) Rosetta pLysS | F′ ompT hsdSB (rB− mB−) gal dcm (DE3)pLysSRARE2 (Camr) | Novagen |
| JR01 | Rosetta pLysS carrying plasmid pJR1 | This work |
| 302Δa | E. coli cysG Nirs Lac+ CysG− | 31 |
| cysG pER247 strain | Strain 302Δa carrying the plasmid pER247 (pACYC184-lacq-Ptac-P. denitrificans cobA) | 31 |
| RP7 | E. coli cysG pER247 containing plasmid pET23a, M. tuberculosis Rv2393 | This work |
| RP8 | E. coli cysG pER247 containing plasmid pET23a, M. smegmatis Rv2393 | This work |
| HB101 | E. coli K-12 F−Δ(gpt-proA)62 leuB1 glnV44 ara-14 galK2 lacY1 hsdS20 rpsL20 xyl-5 mtl-1 recA13 | 4 |
| M. smegmatis strains | ||
| mc2155 | ept-1 | 43 |
| RPMS001 | mc2155ΔsirA::hyg sacB | This work |
| RPMS002 | mc2155ΔRv2393::hyg sacB | This work |
| RPMS004 | RPMS001 carrying plasmid pRP15 (M. smegmatis sirA complementation vector [Table 2]) | This work |
| RPMS005 | RPMS002 carrying plasmid pRP16 (M. smegmatis che1 complementation vector [Table 2]) | This work |