TABLE 2.
Plasmids used in this study
| Plasmid | Description | Reference or source |
|---|---|---|
| p0004S | Cosmid containing the Hygr-SacB cassette | 2 |
| phAE159 | Shuttle phasmid, TM4ts::pYUB328 | J. Kriakov and W. R. Jacobs, unpublished |
| pMV261 | Kanr ColE1 oriM aph Phsp60′ | 44 |
| pMV361 | Integrative vector Hygr | 44 |
| pMS2391 | Recombinant cosmid derived from p0004S containing the M. smegmatis sirA AES | This study |
| pMS2393 | Recombinant cosmid derived from p0004S containing the M. smegmatis Rv2393 AES | This study |
| pSKB4-9His-GST | Derived from pGEX-6P-1; contains an N-terminal His tag upstream of a GST tag | 1 |
| pJR1 | pSKB4-9His-GST carrying the M. tuberculosis sirA gene | This study |
| pJR2 | pJR1 with E. coli cysG cloned downstream of sirA | This study |
| pRP15 | pMV261 carrying the M. smegmatis sirA gene | This study |
| pRP16 | pMV261 carrying the M. smegmatis Rv2393 gene | This study |
| pET23a | Cloning vector containing an N-terminal His tag | Novagen |
| pRP17 | pET23a carrying M. tuberculosis Rv2393 | This study |
| pRP18 | pET23a carrying M. smegmatis Rv2393 | This study |
| pKK E. coli cysG | pKK223.3, an overexpression vector derived from pBR322 with the tac promoter, carrying E. coli cysG | 31 |