TABLE 3.
Primers used in this study
| Primera | Sequence (5′-3′) | Amplified sequence |
|---|---|---|
| TBsirA F | GGAATTCCATATGTCCGCGAAGGAGAACCCC | M. tuberculosis sirA |
| TBsirA R | AAGGAAAAAAGCGGCCGCTCATCGCAGGTCGTCCTCCTCGGCCCGGAT | |
| colicysG F | TTTGCGGCCGCAAACGGCTGCCGGTTAATTACTAAGGGGTTTTTAC | E. coli cysG |
| colicysG R | GGCCTAGGTTAATGGTTGGAGAACCAGTTCAGTTTATC | |
| smegsirA F | TACAGCTGATGCTCGAAGACGAGTACTTCAT | M. smegmatis sirA |
| smegsirA R | CCCAAGCTTTCACGTTGCCTACCTCAAATCCGCTTCGTC | |
| smegRv2393 F | CGCAGCTGGTGACGCTCGTCCTGACCGCACAC | M. smegmatis Rv2393 |
| smegRv2393 R | CCCAAGCTTTCAGCGCATGGCCCTGGCCCGTGCACT | |
| 1 | TTTTTTTTCCATAAATTGGGTAGATGCCGAAGGGCCGGCCCGGTGTGGG | 3′-end-flanking region of M. smegmatis sirA |
| 1′ | TTTTTTTTCCATTTCTTGGGCCGACGGCGTCGAGGCGCTTCCAGATCTC | |
| 2 | TTTTTTTTCCATAGATTGGGGCCCCGAGGAGGGTTTCCAGGTGCATCTG | 5′-end-flanking region of M. smegmatis sirA |
| 2′ | TTTTTTTTCCATCTTTTGGGCCCGCATTCGGTCTTGGACAGCCCGGCCC | |
| 3 | TTTTTTTTCCATAAATTGGGTGCGCAACTTCGTGAAACAACGCGAGGAC | 3′-end-flanking region of M. smegmatis Rv2393 |
| 3′ | TTTTTTTTCCATTTCTTGGAGATTCGGTTCGTTCTGCTCACAGAAC | |
| 4 | TTTTTTTTCCATAGATTGGTCGACCGCTACGAAGAGGCGATCGAGG | 5′-end-flanking region of M. smegmatis Rv2393 |
| 4′ | TTTTTTTTCCATCTTTTGGTATCGGTTTCGTGTTCCAGCACTATGCGGC |
a
F and R refer to forward and reverse primers.