TABLE 3.
Effects of TetR/ActR/SCO7222 on various reporter plasmids
| Plasmid(s)a | Bioluminescence (RLU)b | Error (±1 SD) |
|---|---|---|
| pCS26-Pac | 318 | 26 |
| pN1lux | 2.19 × 105 | 3.9 × 104 |
| pN1lux + pACYC184 | 1.21 × 105 | 3.4 × 104 |
| pN1lux + pSCO7222 | 187 | 27 |
| pN2lux | 1.09 × 104 | 2.0 × 103 |
| pN2lux + pACYC184 | 1.25 × 104 | 1.0 × 103 |
| pN2lux + pSCO7222 | 271 | 87 |
| pO7223lux | 8.87 × 104 | 2.7 × 103 |
| pO7223lux + pACYC184 | 5.70 × 104 | 5.8 × 103 |
| pO7223lux + pSCO7222 | 262 | 19 |
| pO7223lux + pTetR | 8.22 × 104 | 1.1 × 104 |
| pO7223lux + pActR | 360 | 57 |
| pOactlux + pACYC184 | 3.01 × 104 | 2.4 × 103 |
| pOactlux + pSCO7222 | 1.86 × 104 | 2.4 × 103 |
a
Analysis was carried out using E. coli XL1-Blue as the host.
b
All values are in relative light units (RLU) and represent the average of at least three independent readings.