TABLE 6.
Summary of in vivo and in vitro assay results with the SCO7222 protein
| Operator | Sequencea | Repressionb
|
KD (nM) for SCO7222 protein | |
|---|---|---|---|---|
| SCO7222 protein | ActR | |||
| Oact related | ||||
| Oact | CGCGACCACCGTTCCAC | N | Y | 30 |
| OactA8G | -------G--------- | N | Y | 23 |
| OactG11C | ----------C------ | N | N | NAc |
| OactA5G | ----G------------ | N | N | 15 |
| O7223 related | ||||
| O7223 | CTGGAACGCCGTTCCAG | Y | Y | 2 |
| O7223A5G | ----G------------ | Y | N | 2 |
| O7223C14G | -------------G--- | Y | Y | 2 |
| O7223C15A | --------------A-- | Y | N | 2 |
a
For point mutants, only the specific nucleotide changes made are shown.
b
Y, strong repression; N, either no or weak repression (less than fivefold reduction).
c
Not applicable as no shift was observed even at the highest concentration of protein tested.