TABLE 3.

IgaA- and RcsB-regulated fusions

Insertion(s)a	Gene	Induction ratiob	Protein function and/or features	Putative transcriptional unit	Reference(s)
i36	trg	−129.6	Methyl-accepting chemotaxis protein	trg	53, 81
i59, i70	srfB	−35.4	Unknown	srfABC	93
i74, r50	bapA	5.4	Large protein with repeats	bapABCD	51
r20, r44	yjbH	70.3	Outer membrane lipoprotein	yjbEFGH	29
r52	narH	−12.5	β-Subunit of nitrate reductase A	narGHJI	8, 79, 80
r55, r56	siiE	−3.3	Large secreted protein coded in SPI-4	siiABCDEF	63, 92
r71	dcuB	−1.9	Anaerobic C4-dicarboxylate transporter	dcuBfumB	29, 38
r85	STM2176	−1.9	Glutathione S-transferase	STM2179- STM2178- STM2177- STM2176- STM2175	58
r97	melB	−1.9	Melibiose uptake	melAB	62, 66, 70, 75
r111	yhhJ	−3.3	Transporter, ABC superfamily	yhiHyhhJ	72
r142, r171	srfA	−9.5	Similar to nuclear antigens	srfABC	93
r150	PSLT071	4.5	Unknown	PSLT071	68
r153	yiaD	17.7	Outer membrane lipoprotein	yiaD	29
r172	STM1491	7.4	ABC-type proline/glycine betaine transport, ATPase component	STM1494- STM1493- STM1492- STM1491	58

^aInsertions i36 to i74 were obtained in the first screen (with igaA expressed from a pBAD18 derivative), and insertions r20 to r172 arose from the second screen (with rcsB expressed from a pBAD18 derivative).

^bInduction ratios were determined as follows: igaA5/igaA⁺ for genes positively regulated by RcsB (positive values) and igaA⁺/igaA5 for genes negatively regulated by RcsB (negative values).