FIG. 5.

Activity of HhoAΔN34 against isolated total membrane proteins. Isolated total membranes of WT or ΔhhoA mutant cells were incubated without (lanes −) or with (lanes +) recombinant HhoAΔN34 at 15 and 45°C in 50 mM Tris (pH 7.5) supplemented with 20 mM CaCl₂, separated by SDS-PAGE, and visualized by Coomassie blue staining. The open arrows indicate bands of added HhoAΔN34 and its degradation fragments, and the filled arrows indicate protein bands specifically degraded by HhoAΔN34. Lane M contained markers.