TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Characteristic(s) | Reference or source |
|---|---|---|
| Strains | ||
| Alcaligenes sp. strain NyZ215 | Wild type; ONP+ | This study |
| Alcaligenes sp. strain NyZ216 | Mutant of Alcaligenes sp. strain NyZ215; ONP− | This study |
| P. putida PaW340 | Trp− Strr; plasmid-free derivative of P. putida mt-2, grown on catechol through ortho-cleavage pathway | 13, 39 |
| E. coli DH5α | Host strain | Gibco, BRL |
| E. coli S17-1 λ-pir | Strr; strain for biparental conjugation | 12 |
| Plasmids | ||
| pUC19 | Ampr; cloning and expression vector | Takara |
| pVLT33 | Kmr; RSF1010-lacIq/Ptac hybrid broad-host-range expression vector | 5 |
| pGEM-T | Ampr; vector for cloning Taq polymerase-synthesized PCR fragment | Promega |
| pZWXA | PCR fragment containing onpA cloned into pUC19 | This study |
| pZWXC | PCR fragment containing onpC cloned into pUC19 | This study |
| pZWX33A | PCR fragment containing onpA cloned into pVLT33 | This study |
| pZWX33AB | PCR fragment containing onpAB cloned into pVLT33 | This study |
| pZWXW1 | 5-kb PCR fragment obtained from first cycle of genome walking cloned into pGEM-T | This study |