TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or relevant characteristicsa | Reference(s) |
|---|---|---|
| P. putida strains | ||
| KT2440 | Wild type, prototroph; Cmr Rifr | 1, 32 |
| M1044b | edd:mini-Tn5-Km Kmr Rifr | 14 |
| M1128b | eda:mini-Tn5-Km Kmr Rifr | 14 |
| M438b | gcd:mini-Tn5-Km Kmr Rifr | 14 |
| PSC278b | glk::pCHESIΩ-Km Rifr | 9 |
| KT2440 ptsN | Kmr; P. putida KT2440 with a kanamycin resistance cassette interrupting the ptsN gene | 3 |
| KT2440 crc | Gmr; P. putida KT2440 with a gentamicin resistance cassette interrupting the crc gene | 3 |
| KT2440 crp | Kmr; P. putida KT2440 with a kanamycin resistance cassette interrupting the crp gene | 3 |
| KT2440 cyoB | Tcr; P. putida KT2440 with a tetracycline resistance cassette interrupting the cyoB gene | 38 |
| KT2440 relA | Kmr; P. putida KT2440 with a kanamycin resistance cassette interrupting the relA gene | 44 |
| KT2440(pWW0r PmΩ Sm) | Smr; P. putida KT2440 with a kanamycin resistance cassette interrupting the Pm gene | 26 |
| KT2440(pWW0::μ 21) | Kmr; P. putida KT2440 with a kanamycin resistance cassette interrupting the xylE gene | 26 |
| Plasmids | ||
| pRK600 | Helper plasmid; tra+ mob+ Cmr | 5, 19 |
| pS10 | IncP1 SmrxylR; transcriptional Pu::lacZ::tet fusion | 3 |
| pWW0 | IncP9 mob+ tra+ 3MB+ | 49 |
a
Apr, Cmr, GmR, and Kmr, resistance to ampicillin, chloramphenicol, gentamicin, and kanamycin, respectively.
b
Obtained from the collection of KT2440 mutants available at our institute.