TABLE 4.
Glucokinase activities in wild-type and mutant cells
| Strain | Substrate(s) | Glucokinase activity (nmol/min/mg protein)a |
|---|---|---|
| KT2440(pWW0) | Glucose | 27.3 ± 1.9 |
| KT2440(pWW0) | Glucose + toluene | 16.4 ± 1.9 |
| KT2440(pWW0) | Toluene | 1.3 ± 0.3 |
| KT2440 pstN(pWW0) | Glucose | 30.2 ± 1.4 |
| KT2440 pstN(pWW0) | Glucose + toluene | 16.2 ± 2.7 |
| KT2440 pstN(pWW0) | Toluene | 0.9 ± 0.1 |
| KT2440 crc(pWW0) | Glucose | 27.3 ± 5.4 |
| KT2440 crc(pWW0) | Glucose + toluene | 27.6 ± 1.7 |
| KT2440 crc(pWW0) | Toluene | 1.4 ± 0.2 |
| KT2440(pWW0 Δ Pm) | Glucose | 26.3 ± 0.3 |
| KT2440(pWW0 Δ Pm) | Glucose + toluene | 26.7 ± 0.4 |
| KT2440(pWW0 Δ Pm) | Toluene | 1.5 ± 0.2 |
a
The data are the averages ± standard deviations of at least three independent determinations done in triplicate.