TABLE 5.
Expression of the Pu promoter fused to lacZ in the wild type and glucose mutants growing under different conditionsa
| Strain | β-Galactosidase activity (Miller units) with:
|
||
|---|---|---|---|
| Glucose | Toluene | Glucose + toluene | |
| KT2440(pWW0) | 310 ± 20 | 6,620 ± 150 | 2,060 ± 40 |
| KT2440 gcd(pWW0) | 185 ± 15 | 6,035 ± 125 | 4,710 ± 700 |
| KT2440 glk(pWW0) | 400 ± 30 | 6,775 ± 100 | 3,870 ± 140 |
| KT2440 ptsN(pWW0) | 450 ± 20 | 8,100 ± 200 | 8,550 ± 190 |
| KT2440 crc(pWW0) | 380 ± 40 | 7,700 ± 500 | 5,680 ± 300 |
a
The strains used were transformed with pS10, which carries a Pu:′lacZ fusion and the xylR gene. Assays were done in duplicate, and the data are the averages ± standard deviations of at least three independent assays.