FIG. 3.
Growth of L-form-like cells in the presence of piperacillin. An exponentially growing culture of strain MG1655 in M cefsulodin medium was separated into two parts. At time zero, piperacillin (5 μg/ml) was added to one of them. At the indicated times, the OD600 of each culture was measured (left), and the cultures were diluted in M medium and assayed on M cefsulodin plates (right). Plates were counted after 24 h incubation. Symbols: squares, culture without piperacillin; circles, culture with piperacillin.