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. 2007 Jul 20;189(19):6787–6795. doi: 10.1128/JB.00882-07

TABLE 1.

C. thermocellum Avicel-grown cellulosomal components identified by nano-LC-ESI-MS, ranked by emPAIa

GenInfo identifier Protein (Putative) function or activityc No. of peptide ionsd emPAI emPAI/CipAe Doc1/CipA (mol%)f PProg XC score Coverage (%)h Mol wt (103) Gel band mol wt (103) Reference
125975556 CipA Scaffoldin 42 5.92 1.00 2.2E-12 378 34 196.7 >195 44
125972933 CelK Exoglucanase (GH9) 39 4.12 0.70 11.0 2.0E-12 350 35 100.6 95-106 23
125974579 CelS Exoglucanase (GH48) 29 3.56 0.60 9.4 6.9E-10 240 32 83.5 75-81 44
125973097 CelR Endoglucanase (GH9) 28 3.19 0.54 8.5 9.3E-11 250 31 82.1 81-86 50
125975557 OlpB Cell-surface anchor 27 2.75 0.47 3.4E-13 210 26 248.0 165-195 31
125973339 GH5 15 2.59 0.44 6.9 1.3E-09 140 25 63.0 59-63
125972791 CelA Endoglucanase (GH8) 14 2.46 0.41 6.4 1.1E-08 120 22 52.6 52-59 16
125973315 CelE Endoglucanase (GH5), CE2 24 2.24 0.38 6.0 9.1E-13 200 32 90.2 86-95 18
125973142 CelJ Endoglucanase (GH9), GH44 42 2.16 0.37 5.8 2.4E-13 390 24 178.0 165-195 16
125974342 XynC Xylanase (GH10) 18 1.57 0.26 4.1 2.0E-10 160 24 69.5 81-95 16
125974464 XynZ Xylanase (GH10), CE1 18 1.57 0.26 4.1 2.0E-09 150 20 92.2 63-70 50
125972934 CbhA Exoglucanase (GH9) 30 1.51 0.26 4.1 3.2E-11 280 22 137.0 133-165 54
125975294 CelT Endoglucanase (GH9) 14 1.29 0.22 3.4 6.0E-11 120 21 68.5 59-70 27
125975353 CelG Endoglucanase (GH5) 9 1.15 0.20 3.1 1.1E-07 90 16 63.2 81-85 30
125975452 XynA Xylanase (GH11), CE4 12 1.15 0.20 3.1 1.6E-08 100 14 74.4 47-52 17
125973912 XghA Xyloglucanase (GH74) 21 1.13 0.19 3.0 3.4E-10 180 22 92.3 86-95 50
125973263 GH9 15 0.94 0.16 2.5 7.4E-08 140 17 82.1 85-95
125973062 CelF Endoglucanase (GH9) 12 0.90 0.15 2.4 3.3E-08 120 19 82.0 81-85 34
125973254 -b Cell-surface anchor 21 0.82 0.14 5.6E-09 178 19 140.5 133-165
125974678 GH5 11 0.71 0.12 1.9 4.0E-10 100 9.3 103.1 106-133
125972735 LicBb Lichenase (GH16) 4 0.62 0.11 1.7 4.1E-07 28.2 8.4 37.9 <47 49
125975293 ManAb Mannanase (GH26) 7 0.61 0.10 1.6 7.5E-10 70.2 19 67.0 63-70 15
125973143 CelQb Endoglucanase (GH9) 9 0.58 0.10 1.6 1.0E-13 70.2 14 79.8 70-81 2
125972556 GH26 5 0.53 0.09 1.4 1.2E-07 40.2 5.8 67.3 63-70
125973055 CelB Endoglucanase (GH5) 5 0.53 0.09 1.4 6.1E-08 50.2 7.6 63.9 63-70 7
125975558 Orf2p Cell-surface anchor 7 0.50 0.08 5.9E-08 70.2 15 74.9 86-95 8
125972796 -b GH9 5 0.49 0.08 1.3 3.7E-06 50.2 11 62.6 59-63
125975243 GH9 8 0.44 0.07 1.1 6.7E-06 80.2 9.3 80.2 85-86
125972926 GH5 3 0.33 0.06 0.9 1.7E-06 30.1 6.3 59.9 59-63
125972567 CelNb Endoglucanase (GH9) 4 0.27 0.05 0.8 3.1E-06 40.2 5.7 82.1 85-86 50
125973343 CelD Endoglucanase (GH9) 4 0.23 0.04 0.6 6.2E-06 30.2 5.2 72.4 59-70 21
125972954 GH9 4 0.21 0.04 0.6 7.3E-06 40.2 2.9 89.4 95-106
125972792 ChiA Chitinase (GH18) 2 0.20 0.03 0.5 8.9E-08 20.2 4.8 55.4 47-52 48
125973914 GH53 2 0.17 0.03 0.5 5.5E-07 20.1 6.0 47.0 <47
125973158 -b Endopygalactorunase 2 0.16 0.03 0.5 7.0E-07 20.2 5.2 64.5 59-63
a

Proteins in boldface are those that have never been observed experimentally in purified cellulosomes previous to this study.

b

Found only in the Avicel sample.

c

CE, carbohydrate esterase family.

d

Number of unique parent peptide ions matched (including different charge states, modifications).

e

emPAI normalized to the value obtained for CipA.

f

Molar percentage per CipA for Doc1-containing subunits, calculated as 100 × (emPAI/CipA)/[Σ(emPAI/CipA)docking subunits].

g

Probability of finding a match as good or better than the observed match by chance.

h

Percentage of amino acid coverage to the matched protein.