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. 2007 Jul 20;189(19):6787–6795. doi: 10.1128/JB.00882-07

TABLE 2.

C. thermocellum cellobiose-grown cellulosomal components identified by nano-LC-ESI-MS, ranked by emPAIa

GenInfo identifier Protein (Putative) function or activityc No. of peptide ionsd emPAI emPAI/CipAe Doc1/CipA (mol%)f PProg XC score Coverage (%)h Mol wt (103) Gel band mol wt (103) Reference
125974464 XynZ Xylanase (GH10), CE1 25 2.70 1.25 14.0 4.0E-13 200 31 92.2 86-95 50
125975556 CipA Scaffoldin 25 2.16 1.00 1.5E-10 230 30 196.7 >195 44
125975452 XynA Xylanase (GH11), CE4 17 1.97 0.91 10.2 1.9E-11 170 31 74.4 59-85 17
125974342 XynC Xylanase (GH10) 16 1.31 0.61 6.8 8.1E-11 160 20 69.5 63-70 16
125972791 CelA Endoglucanase (GH8) 9 1.22 0.56 6.3 7.2E-10 90.2 22 52.6 52-59 16
125973912 XghA Xyloglucanase (GH74) 22 1.21 0.56 6.3 1.6E-10 210 27 92.3 86-95 50
125973339 GH5 9 1.15 0.53 5.9 3.2E-05 80.2 13 63.0 59-63
125972933 CelK Exoglucanase (GH9) 18 1.12 0.52 5.8 6.7E-09 180 21 100.6 95-106 23
125973315 CelE Endoglucanase (GH5), CE2 14 0.99 0.46 5.1 1.1E-12 120 20 90.2 86-95 18
125972556 GH26 8 0.98 0.45 5.0 1.4E-08 60.2 9.5 67.3 63-70
125973055 CelB Endoglucanase (GH5) 7 0.82 0.38 4.2 6.4E-07 70.2 15 63.9 63-70 7
125975557 OlpB Cell-surface anchor 11 0.71 0.33 1.2E-10 90.2 14 248.0 165-195 31
125974678 GH5 10 0.63 0.29 3.2 7.6E-07 88.2 11 103.1 106-133
125975073 XynDb Xylanase (GH10) 7 0.58 0.27 3.0 4.7E-07 70.2 15 71.6 70-75 50
125975353 CelG Endoglucanase (GH5) 5 0.53 0.25 2.8 3.9E-08 50.1 9.2 63.2 63-70 30
125973097 CelR Endoglucanase (GH9) 8 0.51 0.23 2.6 9.6E-08 90.2 8.6 82.1 81-85 50
125973062 CelF Endoglucanase (GH9) 7 0.45 0.21 2.3 1.3E-05 70.2 6.4 82.0 81-85 34
125972934 CbhA Exoglucanase (GH9) 11 0.40 0.19 2.1 5.5E-07 108 8.6 137.0 133-165 54
125972926 GH5 3 0.33 0.15 1.7 1.6E-07 30.1 6.5 59.9 59-63
125973786 -b GH43 5 0.32 0.15 1.7 2.9E-06 50.1 7.8 74.5 63-75
125975243 GH9 6 0.31 0.14 1.6 1.7E-06 60.2 6.5 80.2 85-86
125975294 CelT Endoglucanase (GH9) 4 0.27 0.12 1.3 3.6E-05 40.2 6.5 68.5 59-63 27
125973263 GH9 5 0.25 0.11 1.2 8.9E-08 50.2 6.6 82.1 85-86
125974579 CelS Exoglucanase (GH48) 4 0.23 0.11 1.2 9.8E-08 40.1 5.4 83.5 75-81 44
125972792 ChiA Chitinase (GH18) 2 0.20 0.09 1.0 7.3E-08 20.1 2.5 55.4 47-52 48
125973142 CelJ Endoglucanase (GH9), GH44 6 0.18 0.08 0.9 1.3E-07 54.2 3.4 178.0 165-195 16
125973914 GH53 2 0.17 0.08 0.9 5.0E-05 20.1 6.0 47.0 <47
125972954 GH9 3 0.15 0.07 0.8 6.3E-05 28.2 3.3 89.4 95-106
125972540 -b GH43, α-l-arabinofuranosidase B 3 0.15 0.07 0.8 1.0E-05 30.2 4.1 79.0 63-75
125975558 Orf2p Cell-surface anchor 2 0.12 0.06 5.5E-05 20.2 1.9 74.9 85-95 8
125973343 CelD Endoglucanase (GH9) 2 0.11 0.05 0.6 2.8E-06 20.2 1.8 72.4 59-63 21
125973822 SdbAb Cell-surface anchor 2 0.10 0.05 3.9E-05 20.2 4.6 68.6 63-70 29
125974626 -b GH30, α-l-arabinofuranosidase B 2 0.09 0.04 0.4 1.4E-06 20.2 2.7 110.6 106-133
125973429 XynYb Xylanase (GH10), CE1 2 0.07 0.03 0.3 9.5E-06 20.2 1.2 119.6 106-133 42
a

Proteins in boldface are those that have never been observed experimentally in purified cellulosomes previous to this study.

b

Found only in the cellobiose sample.

c

CE, carbohydrate esterase family.

d

Number of unique parent peptide ions matched (including different charge states, modifications).

e

emPAI normalized to the value obtained for CipA.

f

Molar percentage per CipA for Doc1-containing subunits, calculated as 100 × (emPAI/CipA)/[Σ(emPAI/CipA)docking subunits].

g

Probability of finding a match as good or better than the observed match by chance.

h

Percentage of amino acid coverage to the matched protein.