TABLE 3.
GeneInfo identifier | Protein name or type | Fractional difference in expression
|
Overall change on Avicel | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
14N Avicel/15N Avicel
|
14N cellobiose/15N Avicel
|
14N Avicel/14N cellobiose
|
||||||||||
Ratio 1 | SD | No. of peptides | Ratio 2 | SD | No. of peptides | Ratio 1/ratio 2 | Pb | CipAc | SEd | |||
125975557 | OlpB | 1.404 | 0.299 | 80 | 0.134 | 0.016 | 7 | 10.453 | <0.0001 | 2.063 | 0.672 | Increase |
125975556 | CipA | 1.574 | 0.244 | 179 | 0.311 | 0.047 | 108 | 5.067 | <0.0001 | 1.000 | 0.305 | Increase |
125973339 | GH5 | 1.358 | 0.138 | 14 | 0.314 | 0.031 | 10 | 4.320 | <0.0001 | 0.853 | 0.220 | None |
125974678 | GH5 | 1.171 | 0.131 | 7 | 0.751 | 0.074 | 6 | 1.559 | <0.0001 | 0.308 | 0.081 | Decrease |
125972791 | CelA | 0.939 | 0.112 | 21 | 0.659 | 0.067 | 9 | 1.426 | <0.0001 | 0.281 | 0.075 | Decrease |
125973142 | CelJ | 1.959 | 0.220 | 48 | 0.121 | 0.019 | 3 | 16.191 | <0.0001 | 3.195 | 0.926 | Increase |
125972933 | CelK | 1.776 | 0.353 | 76 | 0.158 | 0.015 | 14 | 11.214 | <0.0001 | 2.213 | 0.680 | Increase |
125975294 | CelT | 1.338 | 0.244 | 14 | 0.197 | 0.011 | 3 | 6.791 | <0.0001 | 1.340 | 0.386 | None |
125973062 | CelF | 1.041 | 0.250 | 18 | 0.161 | 0.007 | 5 | 6.452 | <0.0001 | 1.273 | 0.415 | None |
125972934 | CbhA | 1.030 | 0.180 | 39 | 0.166 | 0.019 | 6 | 6.211 | <0.0001 | 1.226 | 0.369 | None |
125973097 | CelR | 1.441 | 0.325 | 27 | 0.252 | 0.032 | 7 | 5.719 | <0.0001 | 1.129 | 0.380 | None |
125974342 | XynC | 1.105 | 0.102 | 21 | 0.528 | 0.030 | 13 | 2.094 | <0.0001 | 0.413 | 0.100 | Decrease |
125974464 | XynZ | 1.043 | 0.264 | 32 | 4.323 | 1.244 | 48 | 0.241 | <0.0001 | 0.048 | 0.021 | Decrease |
125975452 | XynA | 1.095 | 0.25 | 27 | 2.144 | 0.684 | 32 | 0.511 | <0.0001 | 0.101 | 0.045 | Decrease |
125974579 | CelS | 0.932 | 0.138 | 81 | 0.028 | 0.006 | 4 | 33.274 | <0.0001 | 6.567 | 2.171 | Increase |
125973912 | XghA | 1.035 | 0.151 | 24 | 1.662 | 0.332 | 33 | 0.623 | <0.0001 | 0.123 | 0.040 | Decrease |
125975353 | CelG | 0.947 | 0.245 | 10 | 0.333 | 0.035 | 4 | 2.842 | 0.0004 | 0.561 | 0.198 | Decrease |
125972556 | GH26 | 0.871 | 0.090 | 3 | 1.748 | 0.197 | 5 | 0.499 | 0.0004 | 0.098 | 0.026 | Decrease |
125973315 | CelE | 1.032 | 0.187 | 23 | 0.736 | 0.205 | 9 | 1.401 | 0.0005 | 0.277 | 0.110 | Decrease |
125973263 | GH9 | 1.438 | 0.408 | 12 | 0.546 | 0.061 | 4 | 2.633 | 0.0008 | 0.520 | 0.194 | Decrease |
125973055 | CelB | 0.996 | 0.080 | 4 | 1.320 | 0.231 | 6 | 0.754 | 0.0291 | 0.149 | 0.043 | Decrease |
125972954 | GH9 | 1.565 | 0.245 | 2 | 0.938 | 0.073 | 2 | 1.669 | 0.0742 | 0.329 | 0.091 | None |
125975243 | GH9 | 0.978 | 0.185 | 8 | 1.110 | 0.102 | 6 | 0.881 | 0.1426 | 0.174 | 0.052 | None |
125972792 | ChiA | 1.199 | 0.054 | 2 | 0.774 | 0.253 | 2 | 1.548 | 0.1463 | 0.306 | 0.121 | None |
125973914 | GH53 | 1.372 | 0.257 | 2 | 1.007 | 0.198 | 2 | 1.362 | 0.2528 | 0.269 | 0.093 | None |
Proteins in boldface are those that have never been observed experimentally previous to this study.
P value, probability that the null hypothesis is true, based on a two-tailed Student t test of ratio1 versus ratio 2.
Normalized to the value obtained for CipA.
Standard error was calculated using the simple quotient rule of error propagation, where a protein's ratio on Avicel, its ratio on cellobiose, CipA's ratio on Avicel, and CipA's ratio on cellobiose were considered random and independent.