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. 2007 Jul 20;189(19):6787–6795. doi: 10.1128/JB.00882-07

TABLE 3.

Fractional differences in expression of C. thermocellum Avicel-grown cellulosomal components relative to cellobiose-grown components by RelEx, ranked by P value, and normalized to CipAa

GeneInfo identifier Protein name or type Fractional difference in expression
Overall change on Avicel
14N Avicel/15N Avicel
14N cellobiose/15N Avicel
14N Avicel/14N cellobiose
Ratio 1 SD No. of peptides Ratio 2 SD No. of peptides Ratio 1/ratio 2 Pb CipAc SEd
125975557 OlpB 1.404 0.299 80 0.134 0.016 7 10.453 <0.0001 2.063 0.672 Increase
125975556 CipA 1.574 0.244 179 0.311 0.047 108 5.067 <0.0001 1.000 0.305 Increase
125973339 GH5 1.358 0.138 14 0.314 0.031 10 4.320 <0.0001 0.853 0.220 None
125974678 GH5 1.171 0.131 7 0.751 0.074 6 1.559 <0.0001 0.308 0.081 Decrease
125972791 CelA 0.939 0.112 21 0.659 0.067 9 1.426 <0.0001 0.281 0.075 Decrease
125973142 CelJ 1.959 0.220 48 0.121 0.019 3 16.191 <0.0001 3.195 0.926 Increase
125972933 CelK 1.776 0.353 76 0.158 0.015 14 11.214 <0.0001 2.213 0.680 Increase
125975294 CelT 1.338 0.244 14 0.197 0.011 3 6.791 <0.0001 1.340 0.386 None
125973062 CelF 1.041 0.250 18 0.161 0.007 5 6.452 <0.0001 1.273 0.415 None
125972934 CbhA 1.030 0.180 39 0.166 0.019 6 6.211 <0.0001 1.226 0.369 None
125973097 CelR 1.441 0.325 27 0.252 0.032 7 5.719 <0.0001 1.129 0.380 None
125974342 XynC 1.105 0.102 21 0.528 0.030 13 2.094 <0.0001 0.413 0.100 Decrease
125974464 XynZ 1.043 0.264 32 4.323 1.244 48 0.241 <0.0001 0.048 0.021 Decrease
125975452 XynA 1.095 0.25 27 2.144 0.684 32 0.511 <0.0001 0.101 0.045 Decrease
125974579 CelS 0.932 0.138 81 0.028 0.006 4 33.274 <0.0001 6.567 2.171 Increase
125973912 XghA 1.035 0.151 24 1.662 0.332 33 0.623 <0.0001 0.123 0.040 Decrease
125975353 CelG 0.947 0.245 10 0.333 0.035 4 2.842 0.0004 0.561 0.198 Decrease
125972556 GH26 0.871 0.090 3 1.748 0.197 5 0.499 0.0004 0.098 0.026 Decrease
125973315 CelE 1.032 0.187 23 0.736 0.205 9 1.401 0.0005 0.277 0.110 Decrease
125973263 GH9 1.438 0.408 12 0.546 0.061 4 2.633 0.0008 0.520 0.194 Decrease
125973055 CelB 0.996 0.080 4 1.320 0.231 6 0.754 0.0291 0.149 0.043 Decrease
125972954 GH9 1.565 0.245 2 0.938 0.073 2 1.669 0.0742 0.329 0.091 None
125975243 GH9 0.978 0.185 8 1.110 0.102 6 0.881 0.1426 0.174 0.052 None
125972792 ChiA 1.199 0.054 2 0.774 0.253 2 1.548 0.1463 0.306 0.121 None
125973914 GH53 1.372 0.257 2 1.007 0.198 2 1.362 0.2528 0.269 0.093 None
a

Proteins in boldface are those that have never been observed experimentally previous to this study.

b

P value, probability that the null hypothesis is true, based on a two-tailed Student t test of ratio1 versus ratio 2.

c

Normalized to the value obtained for CipA.

d

Standard error was calculated using the simple quotient rule of error propagation, where a protein's ratio on Avicel, its ratio on cellobiose, CipA's ratio on Avicel, and CipA's ratio on cellobiose were considered random and independent.