FIG. 3.
Decreased ability of the lpp mutant, JG736, to colonize the leech. JG736 was coinoculated with the competitor strain in a 1:1 ratio. The CI [(mutantoutput/competitoroutput)/(mutantinput/competitorinput)] was calculated for each animal and plotted over time. A CI of 1 (dashed line) indicates that the mutant and competitor strain colonize to equal levels. A CI below 1 indicates that the mutant is outcompeted and has a colonization defect. The decrease in the CI is statistically significant from 15 h onward (P < 0.05 by a single-sided, two-tailed t test).